96 Tests PN: B212287

Components:

45x Ab-conjugated beads (S5P9 - Mouse MIP-1γ Ab-bead). PN: B212287A. One vial containing 100 µL of anti-mouse MIP-1γ conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Mouse MIP-1γ Biotin-dAb). PN: B212287B. One vial containing 100 µL of biotinylated anti-mouse MIP-1γ.

Lyophilized Standard Mix - Mouse Group 4 Panel B, 11-Plex. PN: MG4011B. One vial containing lyophilized recombinant 6CKine, Activin A, BDNF, BLC, BRAK, MDC, MIP-1γ, MIP-3β, OPG, TARC, and TIMP-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse MIP-1γ/CCL9/MRP-2/CCF18. Can be multiplexed with other analytes in Mouse Group 4. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage: 2-8 C in the dark

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 4: Negligible

  • Sample volume: 15 µL/test

Description:

CCL9 (Chemokine C-C motif ligand 9) is a small cytokine belonging to the CC chemokine family. It is also called macrophage inflammatory protein-1 gamma (MIP-1γ), macrophage inflammatory protein-related protein-2 (MRP-2) and CCF18 that has been described in rodents. CCL9 has also been previously designated CCL10, although this name is no longer in use. It is secreted by follicle-associated epithelium (FAE) such as that found around Peyer's patches, and attracts dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1.CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption. CCL9 is constitutively expressed in macrophages and myeloid cells.

References:

  1. Zhao X, Sato A, Dela Cruz C, Linehan M, Luegering A, Kucharzik T, Shirakawa A, Marquez G, Farber J, Williams I, Iwasaki A (2003). "CCL9 is secreted by the follicle-associated epithelium and recruits dome region Peyer's patch CD11b+ dendritic cells". J Immunol 171 (6): 2797–803. doi:10.4049/jimmunol.171.6.2797. PMID 12960300.

  2. Lean J, Murphy C, Fuller K, Chambers T (2002). "CCL9/MIP-1gamma and its receptor CCR1 are the major chemokine ligand/receptor species expressed by osteoclasts". J Cell Biochem 87 (4): 386–93. doi:10.1002/jcb.10319. PMID 12397598.

  3. Youn B, Jang I, Broxmeyer H, Cooper S, Jenkins N, Gilbert D, Copeland N, Elick T, Fraser M, Kwon B (1995). "A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors". J Immunol 155 (5): 2661–7. PMID 7650394.

  4. Hara T, Bacon K, Cho L, Yoshimura A, Morikawa Y, Copeland N, Gilbert D, Jenkins N, Schall T, Miyajima A (1995). "Molecular cloning and functional characterization of a novel member of the C-C chemokine family". J Immunol 155 (11): 5352–8. PMID 7594550.