96 Tests PN: A211263

Components:

45x Ab-conjugated beads (S4P12 - Mouse OSM Ab-bead). PN: B211263A. One vial containing 100 µL of anti-mouse OSM conjugated to AimPlex Bead S4P12.

25x Biotin-detection Ab (Mouse OSM Biotin-dAb). PN: B211263B. One vial containing 100 µL of biotinylated anti-mouse OSM.

Lyophilized Standard Mix - Mouse Group 2 Panel B, 8-Plex. PN: MG20008. One vial containing lyophilized recombinant mouse G-CSF, GM-CSF, IL-28A/B, M-CSF, MIP-1α, MIP-1β, OSM, and TSLP.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse OSM/Oncostatin M. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pgmL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Oncostatin M (Accession P53347) is part of the IL-6 cytokine family. It is produced by activated macrophages, monocytes, T cells and dendritic cells. It is most similar to LIF in function and structure and in humans, it even utilizes the LIF receptor in addition to its own. OSM may have fundamental roles regarding inflammation, liver regeneration, and cardiovascular disease. OSM can inhibit growth of solid tumors and melanomas and is identified as a specific cell growth regulator. OSM is produced by both osteocytes and osteoblasts in in mouse bone and is understood to have a role in bone formation and destruction in humans.

References:

  1. Richards. CD. The Enigmatic Cytokine Oncostatin M and Roles in Disease. ISRN Inflammation. 2013; Article ID 512103. Doi: 10.1155/2013/512103.

  2. Tanaka M, Miyajima A. Oncostatin M, a multifunctional cytokine. Rev Physiol Biochem Pharmacol. 2003; 149: 39-52. Doi: 10.1007/s10254-003-0013-1.

  3. Walker EC, McGregor NE, Poulton IJ. Oncostatin M promotes bone formation independently of resorption when signaling through leukemia inhibitory factor receptor in mice. J Clin invest. 2010; 120(2): 582-92. Doi: 10.1172/JCI40568.

  4. Zarling JM, Shoyab M, Marquardt H, Hanson MB, Lioubin MN, Todaro GJ. Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells. Proc Natl Acad Sci USA. 1986; 83(24): 9739-43. Doi: 10.1073/pnas.83.24.9739.