96 Tests PN: C290111
Components:
1x Ab-conjugated beads (premixed). PN: C290111A. One bottle containing 4.7 mL of the following AimPlex antibody-bead conjugates:
S4P3 - anti-M MIP-3beta
S4P4 - anti-M 6Ckine
S4P6 - anti-M IL-1beta
S4P7 - anti-M LIF
S4P11 - anti-M IL-4
S4P12 - anti-M IL-17A
S5P2 - anti-M G-CSF
S5P3 - anti-M IL-1alpha
S5P7 - anti-M IL-13
S5P10 - anti-M IL-2Ra
S5P12 - anti-M GM-CSF
2x Biotin-detection Ab (premixed). PN: C290111B. One vial containing 1.25 mL of the following biotinylated antibodies.
Biotin - anti-M MIP-3beta
Biotin - anti-M 6Ckine
Biotin - anti-M IL-1beta
Biotin - anti-M LIF
Biotin - anti-M IL-4
Biotin - anti-M IL-17A
Biotin - anti-M G-CSF
Biotin - anti-M IL-1alpha
Biotin - anti-M IL-13
Biotin - anti-M IL-2Ra
Biotin - anti-M GM-CSF
2x Mouse/Rat dAb Diluent. PN: 07MRDX21. One vial containing 1.25 mL of biotin detection antibody diluent.
Lyophilized Standard Mix- Mouse Cytokine 11-Plex Panel 1. PN: MG29111. One vial containing lyophilized recombinant 6Ckine, G-CSF, GM-CSF, IL-1alpha, IL-1beta, IL-4, IL-13, IL-17A, sIL-2Ra, LIF and MIP-3beta.
Standard Info Sheet: Indicates reconstitute volume and standard concentrations.
Basic Kit for Premixed Panels:
1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 2.5 mL of 1x SAPE.
10x Reading Buffer. PN: 07RB1. One bottle containing 4 mL of 10x buffer.
10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.
PCR 8-tube Strip. PN: 07P8T1. 2 strips.
Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.
Plate Seals. PN: 07PSR1. 4 sealers.
Application: Optimal antibody pairs and antigen standards for assaying the listed analytes. To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): 6Ckine, < 10 pg/mL; G-CSF, < 5 pg/mL; GM-CSF, < 5 pg/mL; IL-1alpha, < 2 pg/mL; IL-1beta, < 2 pg/mL; IL-4, < 0.5 pg/mL; IL-13, < 5 pg/mL; IL-17A, < 1.5 pg/mL; sIL-2Ra, < 2 pg/mL; LIF < 5 pg/mL; MIP-3beta, < 10 pg/mL.
LLOQs: 6Ckine, < 20 pg/mL; G-CSF, < 10 pg/mL; GM-CSF, < 10 pg/mL; IL-1alpha, < 10 pg/mL; IL-1beta, < 5 pg/mL; IL-4, < 2 pg/mL; IL-13, < 10 pg/mL; IL-17A, < 5 pg/mL; sIL-2Ra, < 5 pg/mL; LIF < 10 pg/mL; MIP-3beta, < 20pg/mL.
ULOQs: 6Ckine, > 5,000 pg/mL; G-CSF, > 5,000 pg/mL; GM-CSF, > 5,000 pg/mL; IL-1alpha, > 5,000 pg/mL; L-1beta, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-13, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; sIL-2Ra, > 5,000 pg/mL; LIF > 5,000 pg/mL; MIP-3beta, > 5,000 pg/mL.
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Negligible
Sample volume: 15 µL/test
For Research Use Only. Not for use in diagnostic procedures.