96 Tests PN: C290181
Components:
1x Ab-conjugated beads (premixed). PN: C290181A. One bottle containing 4.7 mL of the following AimPlex antibody-bead conjugates:
S4P2 - anti-M LIX
S4P3 - anti-M TNFalpha
S4P4 - anti-M MIP-2
S4P5 - anti-M IL-22
S4P6 - anti-M IL-6
S4P7 - anti-M IL-12p70
S4P8 - anti-M IFNgamma
S4P10 - anti-M VEGF
S4P12 - anti-M IL-17A
S5P2 - anti-M G-CSF
S5P3 - anti-M IP-10
S5P4 - anti-M IL-1beta
S5P6 - anti-M MIP-3alpha
S5P7 - anti-M IL-17F
S5P9 - anti-M IL-10
S5P10 - anti-M IL-20
S5P11 - anti-M MIP-1alpha
S5P12 - anti-M GM-CSF
2x Biotin-detection Ab (premixed). PN: C290181B. One vial containing 1.25 mL of the following biotinylated antibodies.
2x Mouse/Rat dAb Diluent. PN: 07MRDX21. One vial containing 1.25 mL of biotin detection antibody diluent.
Biotin - anti-M LIX
Biotin - anti-M TNFalpha
Biotin - anti-M MIP-2
Biotin - anti-M IL-22
Biotin - anti-M IL-6
Biotin - anti-M IL-12p70
Biotin - anti-M IFNgamma
Biotin - anti-M VEGF
Biotin - anti-M IL-17A
Biotin - anti-M G-CSF
Biotin - anti-M IP-10
Biotin - anti-M IL-1beta
Biotin - anti-M MIP-3alpha
Biotin - anti-M IL-17F
Biotin - anti-M IL-10
Biotin - anti-M IL-20
Biotin - anti-M MIP-1alpha
Biotin - anti-M GM-CSF
Lyophilized Standard Mix- Mouse Cytokine 18-Plex Panel 1. PN: MG29181. One vial containing lyophilized recombinant G-CSF, GM-CSF, IFNgamma, IL-1beta, IL-6, IL-10, IL-12p70, IL-17A, IL-17F, IL-20, IL-22, IP-10, LIX, MIP-1alpha, MIP-2, MIP-3alpha, TNFalpha and VEGF.
Standard Info Sheet: Indicates reconstitute volume and standard concentrations.
Basic Kit for Premixed Panels:
1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 2.5 mL of 1x SAPE.
10x Reading Buffer. PN: 07RB1. One bottle containing 4 mL of 10x buffer.
10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.
PCR 8-tube Strip. PN: 07P8T1. 2 strips.
Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.
Plate Seals. PN: 07PSR1. 4 sealers.
Application: Optimal antibody pairs and antigen standards for assaying the listed analytes. To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): G-CSF, < 5 pg/mL; GM-CSF, < 5 pg/mL; IFNgamma, < 0.5 pg/mL; IL-1beta, < 2 pg/mL; IL-6, < 3 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 1 pg/mL; IL-17A, < 1.5 pg/mL; IL-17F, < 5 pg/mL; IL-20, < 10 pg/mL; IL-22, < 5 pg/mL; IP-10, < 3 pg/mL; LIX, < 5 pg/mL; MIP-1alpha, < 5 pg/mL; MIP-2, < 5 pg/mL; MIP-3alpha, < 5 pg/mL; TNFalpha, < 0.5 pg/mL; VEGF, < 5 pg/mL.
LLOQs: G-CSF, < 10 pg/mL; GM-CSF, < 10 pg/mL; IFNgamma, < 2 pg/mL; IL-1beta, < 5 pg/mL; IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-12p70, < 2 pg/mL; IL-17A, < 5 pg/mL; IL-17F, < 10 pg/mL; IL-20, < 20 pg/mL; IL-22, < 10 pg/mL; IP-10, < 10 pg/mL; LIX, < 10 pg/mL; MIP-1alpha, < 10 pg/mL; MIP-2, < 10 pg/mL; MIP-3alpha, < 10 pg/mL; TNFalpha, < 2 pg/mL; VEGF, < 10 pg/mL.
ULOQs: G-CSF, > 5,000 pg/mL; GM-CSF, > 5,000 pg/mL; IFNgamma, > 5,000 pg/mL; IL-1beta, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, 2,000 pg/mL; IL-12p70, 2,000 pg/mL; IL-17A, > 5,000 pg/mL; IL-17F, > 5,000 pg/mL; IL-20, > 5,000 pg/mL; IL-22, > 5,000 pg/mL; IP-10, > 5,000 pg/mL; LIX, > 5,000 pg/mL; MIP-1alpha, > 5,000 pg/mL; MIP-2, > 5,000 pg/mL; MIP-3alpha, > 5,000 pg/mL; TNFalpha, > 1,500 pg/mL; VEGF, > 5,000 pg/mL.
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Negligible
Sample volume: 15 µL/test
For Research Use Only. Not for use in diagnostic procedures.