96 Tests PN: B213461

Components:

45x Ab-conjugated beads (S5P10 - Mouse sTNFRII Ab-bead). PN: B213461A. One vial containing 100 µL of anti-mouse sTNFR2 conjugated to AimPlex Bead S5P10.

25x Biotin-detection Ab (Mouse sTNFRII Biotin-dAb). PN: B213461B. One vial containing 100 µL of biotinylated anti-mouse sTNFR2.

Lyophilized Standard Mix - Mouse Group 5 Panel B, 10-Plex. PN: MG5010B. One vial containing lyophilized recombinant sCD27, sCD105, sIFNγR1, sTNFR1, sTNFRII,  sTWEAK-R, sVEGFR1, sVEGFR2, sIL-1R1, and sRAGE.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse TNFR2/TNFRSF1B/sCD120b. Can be multiplexed with other analytes in Mouse Group 5. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage: 2-8 C in the dark

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 5: Negligible

  • Sample volume: 15 µL/test

Description:

Tumor necrosis factor receptor 2 (TNF-R2), also known as tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). TNFR2 is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A.  TNFR2 and TNF-receptor 1 form a hetero-complex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating anti-oxidative pathways. Diseases associated with TNFRSF1B include palindromic rheumatism and post-transplant lymphoproliferative disease.

References:

  1. Schall TJ, Lewis M, Koller KJ, Lee A, Rice GC, Wong GH, Gatanaga T, Granger GA, Lentz R, Raab H, et al. (June 1990). "Molecular cloning and expression of a receptor for human tumor necrosis factor". Cell 61 (2): 361–70. doi:10.1016/0092-8674(90)90816-W. PMID 2158863.

  2. Santee SM, Owen-Schaub LB (October 1996). "Human tumor necrosis factor receptor p75/80 (CD120b) gene structure and promoter characterization". J Biol Chem 271 (35): 21151–9. doi:10.1074/jbc.271.35.21151. PMID 8702885.

  3. Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.