96 Tests PN: B211269

Components:

45x Ab-conjugated beads (S5P6 - Mouse TSLP Ab-bead). PN: B211269A. One vial containing 100 µL of anti-mouse TSLP conjugated to AimPlex Bead S5P6.

25x Biotin-detection Ab (Mouse TSLP Biotin-dAb). PN: B211269B. One vial containing 100 µL of biotinylated anti-mouse TSLP.

Lyophilized Standard Mix - Mouse Group 2 Panel B, 8-Plex. PN: MG20008. One vial containing lyophilized recombinant mouse G-CSF, GM-CSF, IL-28A/B, M-CSF, MIP-1α, MIP-1β, OSM, and TSLP.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse TSLP. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important : Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pgmL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Thymic Stromal Lymphopoietin (Accession Q9JIE6) is a pro-inflammatory member of the IL-2 cytokine family that activates Langerhans cells. TSLP is secreted by hematopoietic cells and some non-hematopoietic cells such as epithelial cells. In both mouse and human studies, TSLP has been associated with the progression of atopic diseases and keratinocytes and stromal cells have been its primary producers during such events. TSLP has also been implicated in the increased likelihood that people with atopic dermatitis will develop allergic rhinitis and asthma later in life. High expression of TSLP has been found in human lung fibroblasts, bronchial epithelial, and smooth muscle cells, indicating a correlation with respiratory disorders.

References:

  1. Soumelis V, Reche PA, Kanzler H, et al. Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol. 2002; 3(7): 673-80. Doi: 10.1038/ni805.

  2. Ziegler SF. The role of thymic stromal lymphopoietin (TSLP) in allergic disorders. Curr Opin Immunol. 2010; 22(6): 795-9. Doi: 10.1016/j.coi.2010.10.020.

  3. Ziegler SF, Roan F, Bell BD, Stoklasek TA, Kitajima