96 Tests PN: C464402

Components:

1x Ab-conjugated beads (premixed). PN: C464402A. One bottle containing 4.7 mL of the following AimPlex antibody-bead conjugates:

  • S4P5 - anti-NHP IGF I

  • S5P8 - anti-NHP IGF II

2x Biotin-detection Ab(premixed). PN: C464402B. One vial containing 1.25 mL of the following biotinylated antibodies.

  • Biotin - anti-NHP IGF I

  • Biotin - anti-NHP IGF II

2x NR dAb Diluent. PN: 07NDX21. One vial containing 1.25 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- NHP IGF I and IGF II, 2-Plex. PN: NG46402. One vial containing lyophilized recombinant IGF I and IGF II. 

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels: 

  • 1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 2.5 mL of 1x SAPE.

  • 10x Reading Buffer. PN: 07RB1. One bottle containing 4 mL of 10x buffer.

  • 10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.

  • PCR 8-tube Strip. PN: 07P8T1. 2 strips.

  • Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

  • Plate Seals. PN: 07PSR1. 4 sealers.

Application: Optimal antibody pairs and antigen standards for assaying NHP IGF I and IGF II. To be used in conjunction with the AimPlex Basic Kit for Premixed Panels (PN: U100005) and the IGF I/IGF II Diluent Kit (PN: P910102, universal for all sample types). Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Note: Some sample types such as serum and plasma should be pretreated to release IGF I and IGF II from their binding proteins for accurate measurement.  There are number of sample pretreatment methods described in literature including size exclusion chromatography, solid-phase extraction and acid-ethanol extraction.  The acid-ethanol extraction method described by Breier et al. (J Endocrinol. 1991, 128:347-357) has been used in many published studies.  The appropriate specimen pretreatment method should be determined by the investigator according to the sample type and experimental conditions. 

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LODs): IGF I, < 10 pg/mL; IGF II, < 10 pg/mL.

  • LLOQs: IGF I, > 20 pg/mL; IGF II, < 20 pg/mL.

  • ULOQs: IGF I, < 20,000 pg/mL; IGF II, <20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity:               

  • IGF I: Negligible cross-reactivity with IGF II at 10,000pg/mL
    IGF II: < 1% cross-reactivity with IGF I at 10,000pg/mL

  • Sample volume: 15 µL/test

 For Research Use Only.  Not for use in diagnostic procedures.