96 Tests PN: B411145

Components:

45x Ab-conjugated beads (S5P9 - NHP IL-17A Ab-bead). PN: B411145A. One vial containing 100 µL of anti-human IL-17A conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (NHP IL-17A Biotin-dAb). PN: B411145B. One vial containing 100 µL of biotinylated anti-human IL-17A.

Lyophilized Standard Mix-NHP Group 1 Panel A, 7-Plex. PN: NG10007. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

Application: Optimal antibody pair and antigen standard for assaying NHP IL-17A.  Can be multiplexed with other analytes in NHP Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 1 pg/mL

  • Quantitation range:

  • LLOQ: < 3 pg/mL

  • ULOQ: > 1,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in NHP Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 17A is a pro-inflammatory cytokine, secreted by T cells, primarily Th17 cells. IL-17A mediates NF-kappa B activity and can activate the expression of IL-6 and the enzyme prostaglandin-endoperoxide synthase 2. Over expression of IL-17A has been associated with multiple sclerosis, irritable bowel disease, rheumatoid arthritis, asthma, COPD, and ocular diseases. However, there is more recent research to suggest that IL-17A may have beneficial effects on the gut. Active form vitamin D has been linked to impaired production of IL-17 production by Th17 cells.

References:

  1. Chang SH, Chung Y, Dong C. Vitamin D Suppresses Th17 cytokine production by inducing C/EBP homologous protein (CHOP) expression.  J Biol Chem. 2010; 285(50): 38751-5. Doi: 10.1074/jbc.C110.185777.

  2. Monteleone I, Sarra M, Pallone F, Monteleone G. Th-17 related cytokines in inflammatory bowel diseases: friends or foes? Curr Mol Med. 2012; 12(5): 592-7.

  3. Sarra M, Pallone F, Macdonald TT, Monteleon G. IL-23/IL-17 axis in IBD. Inflamm Bowel Dis. 2010; 16(10) 1808-13. Doi: 10.1002/ibd.21248.

  4. Yao Z, et al. Herpesvirus Saimiri encodes a new cytokine IL-17 which binds to a novel cytokine receptor. Immunity 1995; 3(6): 811-21. Doi: 10.1016/1074-7613(95)90070-5.