96 Tests PN: B313286

Components:

45x Ab-conjugated beads (S5P2 - Rat CD154/sCD40L/TRAP/TNFSF5 Ab-bead). PN: B313286A. One vial containing 100 µL of anti-rat CD154/sCD40L/TRAP/TNFSF5 conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (Rat CD154/sCD40L/TRAP/TNFSF5 Biotin-dAb). PN: BB313286B. One vial containing 100 µL of biotinylated anti-rat CD154/sCD40L/TRAP/TNFSF5.

Lyophilized Standard Mix - Rat CD154/sCD40L/TRAP/TNFSF5, PN: B313286S. One vial containing lyophilized CD154/sCD40L/TRAP/TNFSF5.

Application: Optimal antibody pair and antigen standard for assaying Rat CD154/sCD40L/TRAP/TNFSF5. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 50 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Tumor Necrosis Factor Receptor SuperFamily, Member 5 (TNFSF5), also called CD154 or CD40 ligand (CD40L), is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on T follicular helper cells (TFH cells). On TFH cells, CD154 promotes B cell maturation and function by engaging CD40 on the B cell surface and therefore facilitating cell-cell communication. A defect in this gene results in an inability to undergo immunoglobulin class switching and is associated with hyper IgM syndrome. Absence of CD154 also stops the formation of germinal centers and therefore prohibiting antibody affinity maturation, an important process in the adaptive immune system.

References:

  1. Schönbeck U, Libby P (January 2001). "The CD40/CD154 receptor/ligand dyad". Cell. Mol. Life Sci. 58 (1): 4–43. doi:10.1007/PL00000776. PMID 11229815.

  2. Szmitko PE, Wang CH, Weisel RD, de Almeida JR, Anderson TJ, Verma S (October 2003). "New markers of inflammation and endothelial cell activation: Part I". Circulation 108 (16): 1917–23. doi:10.1161/01.CIR.0000089190.95415.9F. PMID 14568885.

  3. Wang JH, Zhang YW, Zhang P, Deng BQ, Ding S, Wang ZK, Wu T, Wang J. (March 2013). "CD40 ligand as a potential biomarker for atherosclerotic instability.". Neurol Res. 35 (7): 693–700. doi:10.1179/1743132813Y.0000000190. PMID 23561892.

  4. Lineberry NB, Su LL, Lin JT, Coffey GP, Seroogy CM, Fathman CG (Aug 2008). "Cutting Edge: The Transmembrane E3 Ligase GRAIL Ubiquitinates the Costimulatory Molecule CD40 Ligand during the Induction of T Cell Anergy". J. Immunol. 181 (3): 1622–6. doi:10.4049/jimmunol.181.3.1622. PMC 2853377. PMID 18641297.