96 Tests PN: B311173

Components:

45x Ab-conjugated beads (S4P7 - Rat IL-12 Ab-bead). PN: B311173A. One vial containing 100 µL of anti-rat IL-12 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (Rat IL-12 Biotin-dAb). PN: B311173B. One vial containing 100 µL of biotinylated anti-rat IL-12.

Lyophilized Standard Mix - Rat Group 1 Panel B, 12-Plex. PN: RG10012. One vial containing lyophilized recombinant rat Eotaxin, G-CSF, GM-CSF, IL-1α, IL-1β, IL-5, IL-12p70, IL-13, KC, IP-10, MIP-1α, MIP-2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Rat IL-12p70. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Rat Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 12 has two subunits, IL-12p35 (Accession Q9R103) and IL-12p40 (Accession Q9R278). Represented in its full name, IL-12p70 has a total molecular weight of 70kDa. IL-12 is a T cell stimulating factor and aids in naïve T cell differentiation into Th1 cells. It also mediates NK cells and CD8+ T cells. IL-12, by increasing production of IFN-γ which in turn increases production of IP-10 exerts anti-angiogenic properties. IL-23 is closely related to IL-12 in structure as it shares the p40 subunit. IL-12 is linked to autoimmune diseases such as psoriasis and inflammatory bowel disease though it may inhibit food allergy susceptibility. It also plays an integral role in the immunity against M. tuberculosis.

References

  1. Kalinski P, Hilkens CM, Snijders A, Sniidwint FG, Kapsenberg ML. IL-12 deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naïve T helper cells. J Immunol 1997; 159(1): 28-35; PMID 9200435.

  2. Hsieh CS, Macatonia SE, Tripp CS, Wolf SD, O’Garra A, Murphy KM. Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages. Science 1993; 260(5107) 547-9. Doi:10.1126/science.8097338.

  3. Hunter CA. New IL-12-family members:IL-23 and IL-27, cytokines with divergent functions. Nature Reviews Immunology. 2005; 5: 521-531. Doi: 10.1038/nri1648.

  4. Robinson CM, Nau GJ. Interleukin-12 and interleukin-27 regulate macrophage control of Mycobacterium tuberculosis. J Infect Dis. 2008; 198(3): 359-366. Doi:10.1086/589774.

  5. Temblay JN, Bertelli E, Arques JL, Regoi M, Niocletti C. Production of IL-12 by Peyer patch-dendritic cells is critical for the resistance to food allergy. J Allergy Clin Immunol. 2007; 120(3): 659-65. Doi:10.1016/j.jaci.2007.04.044.