96 Tests PN: B311177

Components:

45x Ab-conjugated beads (S5P12 - Rat IL-13 Ab-bead). PN: B311177A. One vial containing 100 µL of anti-rat IL-13 conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (Rat IL-13 Biotin-dAb). PN: B311177B. One vial containing 100 µL of biotinylated anti-rat IL-13.

Lyophilized Standard Mix - Rat Group 1 Panel B, 12-Plex. PN: RG10012. One vial containing lyophilized recombinant rat Eotaxin, G-CSF, GM-CSF, IL-1α, IL-1β, IL-5, IL-12p70, IL-13, KC, IP-10, MIP-1α, MIP-2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Rat IL-13. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Rat Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 13 (Accession P42203) is a cytokine primarily secreted by Th2 cells, though many cell types may also secrete it. As IL-13 has 25% sequence homology and shares secondary structural features with IL-4, they have similar effects on hematopoietic cells. IL-13 has anti-inflammatory properties that allow it to function as a mediator to allergic inflammation. IL-13 is important in airway remodeling and mucus production and can induce CC-chemokines in lung tissue. Due to its role in apoptosis and tumor cell growth, IL-13 effector function may have effects on B cell chronic lymphocytic leukemia and Hodgkin’s disease. IL-13 is increasingly being associated with diseases in the gut. It may play a role in innate immune responses and have associations with ulcerative colitis, eosinophilic esophagitis, and fibrosis.

References:

  1. Mannon P, Reinisch W. Interleukin 13 and its role in gut defence and inflammation. Gut. 2012; 61:1765-1773. Doi: 10.1136/gutjnl-2012-303461.

  2. Minty A, et al. Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature. 1993; 362 (6417): 248-50. Doi: 10.1038.362248a0.

  3. Rael EL, Lockey RF. Interleukin-13 Signaling and Its Role in Asthma. World Allergy Organ J. 2011; 4(3): 54-64. Doi: 10.1097/WOX.0b013e31821188e0.

  4. Wynn TA. IL-13 Effector Functions. Annu Rev Immunol. 2003; 21: 425-456. Doi: 10.1146/annurev.immunol.21.120601.141142