96 Tests PN: A311105
Components:
45x Ab-conjugated beads (S5P4 - Rat IL-1α Ab-bead). PN: A311105A. One vial containing 100 µL of anti-rat IL-1α conjugated to AimPlex Bead S5P4.
25x Biotin-detection Ab (Rat IL-1α Biotin-dAb). PN: A311105B. One vial containing 100 µL of biotinylated anti-rat IL-1α.
Lyophilized Standard Mix - Rat Group 1 Panel B, 12-Plex. PN: RG10012. One vial containing lyophilized recombinant rat Eotaxin, G-CSF, GM-CSF, IL-1α, IL-1β, IL-5, IL-12p70, IL-13, KC, IP-10, MIP-1α, MIP-2. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying Rat IL-1α/IL-1F1. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 2 pg/mL
Quantitation range:
LLOQ: < 5 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Rat Group 1: Negligible
Sample volume: 15 µL/test
Description:
Interleukin 1 alpha (Accession P16598), also known as hematopoietin-1 is a member of the Interleukin 1 cytokine family. Originally thought to be just Interleukin-1, IL-1α is in fact a distinct protein from interleukin-1 β. It is a pro-inflammatory cytokine that especially incites fever and sepsis. IL-1α is primarily produced by macrophages, neutrophils, epithelial and endothelial cells. It binds to IL-1 receptor, which also binds to IL-1 receptor antagonist. IL-1α acts as a type of molecular “decision-making nexus” of cells over the severity of damage or infection to their associated tissue. It is also an important cytokine in barrier function of the epidermis during the aging process.
References:
Barland CO, Zettersten E, Brown BS, Ye J, Elias P, Ghadially R. Imiquimod-Induced Interleukin-1α Stimulating Improves Barrier Homeostasis in Aged Murine Epidermis. J Invest Dermatol. 2004; 122(2): 330-336. Doi: 10.1046/j.0022-202X.2004.22203.x.
Di Paolo NC, Shayakhmetov DM. Interleukin 1α and the inflammatory process. Nat Immunol. 2016; 17(8): 906-913. Doi: 10.1038./ni.3503.
March CJ, et al. Cloning, sequence, and expression of two distinct human interleukin-1 complementary DNAs. Nature. 1985; 315(6021) 641-7. Doi: 10.1038/315641a0.