96 Tests PN: A311121

Components:

45x Ab-conjugated beads (S4P4 - Rat IL-4 Ab-bead). PN: A311121A. One vial containing 100 µL of anti-rat IL-4 conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Rat IL-4 Biotin-dAb). PN: A311121B. One vial containing 100 µL of biotinylated anti-rat IL-4.

Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. PN: RG10007. One vial containing lyophilized recombinant rat IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Rat IL-4. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Rat Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin-4 (Accession Q63257) is produced by T-cells, mast cells, and basophils. The interferon IFN-γ and IL-4 are mutually antagonistic in their functions of differentiation and antibody class distinctions. IL-4 stimulates B and T-cell proliferation and differentiation and B-cell class switching from IgM to IgG1 and IgE. IL-4 can cross through the blood-brain barrier and even plays a constructive role in memory and learning within the brain. IL-4 has been demonstrated to drive dedifferentiation, and metastasis in rhabdomyosarcoma. Along with other Th2 cytokines, it is associated with airway inflammation and allergic reactions.

References:

  1. Choi P, Reiser H. IL-4: role in disease and regulation of production. Clin. Exp. Immunol. 1998; 113(3): 317-319. Doi:10.1046/j.1365-2249.1998.00690.x

  2. Gadani SP, Cronk JC, Norris GT, Kipnis J. IL-4 in the brain: a cytokine to remember. J Immunol. 2012;189(9):4213–4219. doi:10.4049/jimmunol.1202246.

  3. Hershey GK, Friedrich MF, Esswein LA, Thomas ML, Chatila TA. The association of atopy with a gain-of-function mutation in the alpha subunit of the interleukin-4 receptor. N. Engl. J. Med. 1997; 337(24): 1720-5. Doi:10.1056.NEJM199712113372403.

  4. Hosoyama T, Aslam MI, Abraham J, Prajapai SI, Nishijo K, Michalek JE, Zarazabal LA, Nelon LD, Guttridge DC, Rubin BP, Keller C. IL-4 Drives Dedifferentiation, Mitogenesis and Metastasis in Rhabdomyosarcoma. Clin. Cancer Res. 2011; 17(9): 2757-2766. Doi:10.1158/1078-0432.CCR-10-3445.