96 Tests PN: B312233

Components:

45x Ab-conjugated beads (S4P4 - Rat MCP-3 Ab-bead). PN: B312233A. One vial containing 100 µL of anti-rat MCP-3 conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Rat MCP-3 Biotin-dAb). PN: B312233B. One vial containing 100 µL of biotinylated anti-rat MCP-3.

Lyophilized Standard Mix - Rat Group 2 Panel B, 7-Plex. PN: RG20007. One vial containing lyophilized recombinant rat LIX, MCP-1, MCP-3, RANTES, SDF-1, ICAM-1, and VCAM-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Rat CCL7/MARC/MCP-3. Can be multiplexed with other analytes in Rat Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Rat Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

CCL7, previously named MCP-3 (Accession Q9QXY8) is a member of the MCP subgroup of CC chemokines. It is produced by tumor cell lines and macrophages. CCL7 attracts neutrophils and monocytes. CCL7 participates in anti-inflammatory responses and can promote tumor progression, though there is research to suggest that it may hinder tumor proliferation under certain circumstances. Beyond cancer in adipose tissue, diseases correlated to the detection of CCL7 include demyelinating disease and nephrogenic systemic fibrosis.

References:

  1. Combadiere C, Ahuja SK, Van Damme J, Tiffany HL, Gao J, Murphy PM. Monocyte Chemoattractant Protein-3 is a Functional Ligand for CC Chemokine Receptors 1 and 2B. J. Bio. Chem. 1995; 270: 29671-29675. Doi:10.1074/jbc.270.50.29671.

  2. Liu Y, Cai Y, Liu L, Wu Y, Xiong X. Crucial biological functions of CCL7 in cancer. Peer J 6:e4928. 2018. Doi: 10.7717/peerj.4928.

  3. McQuibban GA, Gong JH, Tam EM, McCulloch CA, Clark-Lewis I, Overall CM. Inflammation dampened by gelatinase A cleavage of monocyte chemoattractant protein-3. Science. 2000; 289(5482): 1202-6. Doi:10.1126/science.289.5482.1202.