96 Tests PN: B315313

Components:

45x Ab-conjugated beads (S5P2 - Rat TIMP-1 Ab-bead). PN: B315313A. One vial containing 100 µL of anti-rat TIMP-1 conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (Rat TIMP-1 Biotin-dAb). PN: B315313B. One vial containing 100 µL of biotinylated anti-rat TIMP-1.

Lyophilized Standard Mix - Rat TIMP-1. PN: B315313S. One vial containing lyophilized TIMP-1.

Application: Optimal antibody pair and antigen standard for assaying Rat TIMP-1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

TIMP metallopeptidase inhibitor 1 (TIMP-1), also known as TIMP1, is a glycoprotein that is expressed from the several tissues of organisms. TIMP-1 protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Diseases associated with TIMP1 include oral submucous fibrosis and Sorsby Fundus Dystrophy.

References:

  1. Reichenstein M, Reich R, LeHoux JG, Hanukoglu I (February 2004). "ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells". Mol. Cell. Endocrinol. 215 (1-2): 109–14. doi:10.1016/j.mce.2003.11.011. PMID 15026182.

  2. Ma J (Dec 2013). "Upregulated TIMP-1 correlates with poor prognosis of laryngeal squamous cell carcinoma.". Int J Clin Exp Pathol. 15 (1): 246–54. PMC 3885479. PMID 24427345.

  3. Tarhini AA (Jan 2014). "A four-marker signature of TNF-RII, TGF-α, TIMP-1 and CRP is prognostic of worse survival in high-risk surgically resected melanoma..". J Transl Med. 12. doi:10.1186/1479-5876-12-19. PMC 3909384. PMID 24457057.